Contact an Account ManagerSeparation of dyes (buffered method)
Summary
Type: HPLC
Application #: 123990
Catalog#: 760326.30 - MN HPLC Column, Nucleodur C18 HTec, 100A, 3um, 3x250mm
Detection:
Conditions: Eluent: A) methanol; B) 25 mmol/L KH2PO4, pH 7
Gradient: 37 % B for 3 min, 37-30 % B in 4 min, 30-10 % B in 8 min, 10-0 % B
in 10 min, 0 % B for 5 min
Flow rate: 0.7 mL/min
Temperature: 60 °C
Injection volume: 1.0 µL
Authors: HPLC department
Source: Macherey-Nagel, Germany 2010
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